Research Article
Effects of Guchang Capsule on Dextran Sulphate Sodium-Induced Experimental Ulcerative Colitis in Mice
Table 1
List of primers used in the study.
| | Number | Gene | Primer sequence | Primer length |
| | 1 | TNFα | Forward 5′-GCCACCACGCTCTTCTGTCT-3′
Reverse 5′-TGAGGGTCTGGGCCATAGAAC-3′ | 20 bp
21 bp | | 2 | IL-6 | Forward 5′-ACAACCACGGCCTTCCCTAC-3′
Reverse 5′-CATTTCCACGATTTCCCAGA-3′ | 20 bp
20 bp | | 3 | IL-1β | Forward 5′-ACCTTCCAGGATGAGGACATGA-3′
Reverse 5′-AACGTCACACACCAGCAGGTTA-3′ | 22 bp
22 bp | | 4 | GAPDH | Forward 5′-ACCACAGTCCATGCCATCAC-3′
Reverse 5′-TCCACCACCCTGTTGCTGTA-3′ | 20 bp
20 bp | | 5 | lncRNA Lethe | Forward 5′-CAGGACTGAGGAGGACATCA-3′
Reverse 5′-GCCTCTCTTCTCAGCGTTGT-3′ | 20 bp
20 bp | | 6 | lncRNA-Cox2 | Forward 5′-AAGGAAGCTTGGCGTTGTGA-3′
Reverse 5′-GAGAGGTGAGGAGTCTTATG-3′ | 20 bp
20 bp | | 7 | lncRNA AS-IL1α | Forward 5′-AGCTCCCATGGATGCCTTTAG-3′
Reverse 5′-GCCCTAAGAACAGGGCTAGG-3′ | 21 bp
20 bp | | 8 | lncRNA NKILA | Forward 5′-GAAACAGGTGCACGTTTCAGG-3′
Reverse 5′-ACAGATGTACCCTGGCAACC-3′ | 21 bp
20 bp | | 9 | lncRNA THRIL | Forward 5′-AACTCCTGACCTCAGGTGATCCAT-3′
Reverse 5′-AAGGGAGTTTCAGAAGGTGTGGCT-3′ | 24 bp
24 bp | | 10 | lncRNA-IL7R | Forward 5′-CCAGCCTTTGCCTCTTCCTTCAAT-3′
Reverse 5′-CCGTACCAAGTCTCTTAGCCCCTC-3′ | 24 bp
23 bp |
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