Cytotoxicity and Genotoxicity Assessment of Sandalwood Essential Oil in Human Breast Cell Lines MCF-7 and MCF-10A

Figure 7

Sandalwood essential oil induces protein expression of Ku70, Ku80, EPHX1, and 14-3-3ζ in MCF-7 cells. (a) Western blot analysis from 25 µg of protein extracted from MCF-7 cells treated with SEO for 5 min, 1 hr, 5 hrs, and 24 hrs. GAPDH was used as loading control. Densitometric quantification of (b) Ku70 shows the highest induction of the protein at 24 hours, while (c) Ku80, (d) EPHX1, and (e) 14-3-3ζ achieve their highest induction at 1 hr of exposure. Asterisk () denotes statistical significance when compared with the control (Student’s -test). Each bar represents the mean of three independent experiments (mean ± SEM).

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