Anti-Inflammatory Activity of Bee Venom in BV2 Microglial Cells: Mediation of MyD88-Dependent NF-κB Signaling Pathway

Figure 2

Bee venom inhibits lipopolysaccharide- (LPS-) induced nitric oxide (NO) production in BV2 microglia. (a) The effect of bee venom on the LPS-induced NO production in BV2 cells. (b) The effect of bee venom on the cell viability of LPS-stimulated BV2 cells. BV2 microglial cells were pretreated with different concentrations of bee venom extract (0.625 μg/mL–2.5 μg/mL) for 30 min and then incubated with LPS (0.1 μg/mL) for 24 h. NO production was determined in the cell supernatants as described in Section 2. After determination of NO production, the viability was immediately examined using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay as described in Section 2. The data are presented as mean ± standard error (SEM), and the experiments were repeated three to five times. versus LPS alone. versus basal.

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