Guizhi Fuling Wan, a Traditional Chinese Herbal Formula, Sensitizes Cisplatin-Resistant Human Ovarian Cancer Cells through Inactivation of the PI3K/AKT/mTOR Pathway

Figure 3

P-gp function changes in SKOV3/DDP cells after treatment for 24 h with verapamil or the rat medicated sera containing GFW corresponding to oral doses by flow cytometric analyses. (a) Representative flow cytometry overlay plot is shown. The P-gp inhibition effect increased along with the peaks moving far away from the vertical-axis. (b) Bar graph of mean fluorescence intensities (MFIs) of Rho123 in SKOV3/DDP cells with different treated groups. The results are the mean ± SD of 3 independent experiments. , ; NS, no difference. LD sera represent the rat medicated sera corresponding to the GFW dosage of 4 gkg⁻¹d⁻¹. MD sera represent the rat medicated sera corresponding to the GFW dosage of 8 gkg⁻¹d⁻¹. HD sera represent the rat medicated sera corresponding to the GFW dosage of 16 gkg⁻¹d⁻¹.

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