Chinese Herbal Medicine Fuzheng Kang-Ai Decoction Inhibited Lung Cancer Cell Growth through AMPKα-Mediated Induction and Interplay of IGFBP1 and FOXO3a

Figure 5

FZKA decoction induced protein levels of IGFBP1 and FOXO3a through the AMPKα pathway. (a-b) A549 (a) and PC9 (b) cells were exposed to increased concentration of FZKA decoction for 24 h. Afterwards, the expressions of IGFBP1 and FOXO3a proteins were detected by Western blot. (c-d) A549 (c) and PC9 (d) cells were treated with Compound C (10 μM) for 2 h before exposure of the cells to FZKA decoction (20 mg/mL) for an additional 24 h. Afterwards, the phosphorylation of AMPKα and expression of IGFBP1 and FOXO3a proteins were detected by Western blot using antibodies against FOXO3a and IGFBP1. The bar graphs represent the mean ± SD of IGFBP1/GAPDH and FOXO3a/GAPDH of three independent experiments. Significant difference from untreated control cells Significant difference from FZKA decoction treated alone ().

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