Chinese Herbal Medicine Fuzheng Kang-Ai Decoction Inhibited Lung Cancer Cell Growth through AMPKα-Mediated Induction and Interplay of IGFBP1 and FOXO3a

Figure 6

Silencing of IGFBP1 reversed the effect of FZKA decoction on cell growth inhibition and abolished the induction of FOXO3a protein expression. (a) A549 cells were transfected with control or IGFBP1 siRNAs with Lipofectamine RNAiMAX reagent for 24 h, followed by exposure of the cells to FZKA decoction (20 mg/mL) for an additional 24 h. Afterwards, the cells proliferation was detected using MTT assays. The expression of IGFBP1 protein was determined by Western blot. (b-c) A549 (b) and PC9 (c) were transfected with control or IGFBP1 siRNAs with Lipofectamine RNAiMAX reagent for 24 h, followed by exposure of the cells to FZKA decoction (20 mg/mL) for an additional 24 h. Afterwards, the expression of IGFBP1 and FOXO3a proteins was determined by Western blot. The bar graphs represent the mean ± SD of FOXO3a/GAPDH of three independent experiments. Significant difference from untreated control cells Significant difference from FZKA decoction treated alone ().

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