Chinese Herbal Medicine Fuzheng Kang-Ai Decoction Inhibited Lung Cancer Cell Growth through AMPKα-Mediated Induction and Interplay of IGFBP1 and FOXO3a

Figure 7

Silencing of FOXO3a overcame FZKA decoction-inhibited cell growth; overexpression of FOXO3a strengthened the effect of FZKA decoction on IGFBP1 expression and phosphorylation of AMPKα. (a-b) A549 cells were transfected with control or FOXO3a siRNAs with Lipofectamine RNAiMAX reagent for 24 h, followed by exposure of the cells to FZKA decoction (20 mg/mL) for an additional 24 h. Afterwards, the cells proliferation and expression of FOXO3a and IGFBP1 proteins were detected using MTT assays and Western blot. (c-d) Cells were transfected with control (pEGFP-N1) or FOXO3a (FOXO3a-pEGFP) expression vector for 24 h before exposing the cells to FZKA decoction for an additional 24 h. Afterwards, the expression of FOXO3a and IGFBP1 proteins (c) and phosphorylation of AMPKα (d) were detected by Western blot. Data are expressed as a percentage of total cells. Values in bar graphs were given as the mean ± SD from three independent experiments performed in triplicate. Significant difference as compared to the untreated control group (). Significant difference from FZKA decoction treated alone ().

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