Chinese Herbal Medicine Fuzheng Kang-Ai Decoction Inhibited Lung Cancer Cell Growth through AMPKα-Mediated Induction and Interplay of IGFBP1 and FOXO3a

Figure 8

The effect of FZKA decoction treatment in orthotopic mice model. (a) The xenografts were assessed by in vivo bioluminescence imaging at the end of the experiments (on day 30). The tumor growth was monitored by injecting luciferin in the mice followed by measuring bioluminescence using IVIS Imaging System. Imaging and quantification of signals were controlled by the acquisition and analysis software living image as described in Section 2. Representative images are shown. ((b) and (c)) The xenografts were harvested on day 30, and the volume and weight of tumors were measured. The bar graphs represented the tumor weight and volume of mice results of mean ± SD. Significant difference from untreated control (). (d) At the end of the experiments, xenograft tumors were isolated and the corresponding lysates were processed for detecting IGFBP1 and FOXO3a proteins and phosphorylation of AMPKα by Western blot. GAPDH was used as loading control. The bar graphs represented the tumor weight and volume of mice results of mean ± SD. Significant difference from untreated control group (). (e) The schematic diagram shows that FAKA decoction inhibits growth of NSCLC cells through AMPKα-mediated increase in FOXO3a and IGFBP1 proteins. Moreover, exogenous expression of FOXO3a feedback strengthened FZKA decoction-induced IGFBP1 and phosphorylation of AMPKα. Thus, the reciprocal interplay of IGFBP1 and FOXO3a contributes to the overall response of FAKA decoction.

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