Berberine Inhibits Intestinal Polyps Growth in Apc (min/+) Mice via Regulation of Macrophage Polarization

Figure 1

Berberine treatment leads to alteration in the proportion and distribution of the macrophages in the intestinal polyps. (a) Tumor numbers were counted in the small intestine and colon of Apc (min/+) mice, . (b) Immunohistochemistry staining of F4/80, iNOS, and MR in the small intestine was performed. Five images were randomly selected and the cell numbers were calculated. Bar = 100 μm. (c) Total mRNA was extracted and mRNA expression level was measured by real-time PCR, . (d) Fluorescent immunocytochemistry was performed to assess protein expression of iNOS, MR, and F4/80. Bar = 200 μm. .

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