Salvianolate Protects Hepatocytes from Oxidative Stress by Attenuating Mitochondrial Injury

Figure 3

Protective effects of Salvianolate on CCl₄-induced liver injury in rats. Male Wistar rats were treated as described in the legend. Serum ALT (b), AST (c), and TBA (d) were assessed by liver function tests kits, respectively. Levels of serum liver functions were decreased, and HSI (a) was alleviated by Salvianolate treatment with dose of 40 mg/kg in CCl₄-induced liver injury mice. (e) Histologic evaluation of liver tissues was stained with H&E, and the expression of Cyto-C and 4-HNE was assayed by IHC (×200). Semiquantitative analysis of liver injury was performed according to the nonalcoholic fatty liver disease activity score (NAS): (f) hepatic steatosis: 0 (<5%); 1 (5%–33%); 2 (34%–66%); and 3 (>66%) and (g) ballooning degeneration of liver cell: 0, none; 1, rare; and 2, many. , versus normal control; and , versus model control.

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