Research Article
Flavonoids Extraction from Propolis Attenuates Pathological Cardiac Hypertrophy through PI3K/AKT Signaling Pathway
Figure 6
FP prevents isoproterenol-induced cardiac remodeling in vivo, which is correlated with PI3K/AKT signaling. (a-b) Intragastric injection of FP (25 mg·kg−1·d−1) or saline (CT) for 7 days was followed by continuous infusion with isoproterenol (ISO, 25 mg·kg−1·d−1) for 7 days to experimentally induce heart hypertrophy. The animals were euthanized and the hearts were removed for hypertrophic evaluation. (a) Representative Western blotting assays and quantitative analysis of phosphorylated AKT in the hearts by drug treatment ( compared to the control group; compared to the isoproterenol group). (b) Effect of a selective PI3K antagonist, wortmannin (WM), on isoproterenol-induced gene reactivation. The heart tissues were collected and assayed for ANF and β-MHC expression. The results are expressed as the means ± SE; mice per group ( compared to the isoproterenol group; compared to the FP-isoproterenol cotreated group).
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