In Vitro Anticancer Activity of a Nonpolar Fraction from Gynostemma pentaphyllum (Thunb.) Makino

Figure 3

EA1.3A induces apoptosis in MDA-MB-453 cells. After treatment with EA1.3A, breast cancer cells MDA-MB-453 were stained with FITC Annexin V/PI and apoptosis was quantified by flow cytometry. (a) Dose effect of EA1.3A treatment (72 hours) on apoptosis. Average values are from three independent experiments (). (b) Time course of EA1.3A treatment (50 μg/mL) on apoptosis. Average values are from three independent experiments (). (c) Western blotting analysis of apoptosis marker cleaved nuclear poly(ADP-ribose) polymerase (cPARP). Data are shown as mean ± SD.

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