Preliminary Screening of Antioxidant and Antibacterial Activities and Establishment of an Efficient Callus Induction in Curculigo latifolia Dryand (Lemba)

Table 3

Inhibition effect of 10 g L⁻¹ of C.latifolia ethanoic extracts (leaf and tuber in vitro and in vivo generated) against the growth of four pathogenic bacteria.

	Tested microorganisms	Types of plants	Inhibitory zone (mm) ± standard deviation	Ampicillin (30 g) ± standard deviation
Gram-positive bacteria	S. aureus	Leaf extracts in vitro	7.1 ± 1.54	18 ± 2.84
		Tuber extract in vitro	12.1 ± 1.14	18 ± 2.57
		Tuber extract in vivo	13.8 ± 1.24	18.2 ± 1.14
		Leaf extracts in vivo	7.8 ± 1.47	18.7 ± 2.64
	Bacillus cereus	Leaf extracts in vitro	7.0 ± 1.30	25 ± 3.60
		Tuber extract in vitro	12.5 ± 1.10	25 ± 3.10
		Tuber extract in vivo	17.3 ± 1.24	25 ± 3.10
		Leaf extracts in vivo	7.2 ± 1.10	25 ± 1.60
Gram-negative bacteria	Klebsiella sp.	Leaf extracts in vitro	7.1 ± 1.30	24 ± 3.20
		Tuber extract in vitro	17.4 ± 1.10	24 ± 4.30
		Tuber extract in vivo	22.3 ± 1.24	24 ± 3.20
		Leaf extracts in vivo	7.4 ± 1.10	24 ± 3.20
	P. aeruginosa	Leaf extracts in vitro	10.2 ± 1.50	31 ± 2.10
		Tuber extract in vitro	18.8 ± 1.7	30.1 ± 1.80
		Tuber extract in vivo	25.4 ± 1.01	30.1 ± 1.80
		Leaf extracts in vivo	10.7 ± 1.10	30.2 ± 1.10

Inhibition zone in mm (5 mm diameter of disk) as the means of triplicate of experiments. The data were analysed by one-way ANOVA and the inhibition means of samples were compared using Duncan’s multiple comparison test (DMCT). Different letters in the same column represent a significant difference at the 5% level in Duncan’s multiple range tests.