Ilexonin A Promotes Neuronal Proliferation and Regeneration via Activation of the Canonical Wnt Signaling Pathway after Cerebral Ischemia Reperfusion in Rats

Figure 1

Effect of IA on NSCs proliferation and neuronal regeneration in peripheral ischemic tissue after ischemia reperfusion. (a) Viable NSCs proliferation detected by double-labeling immunofluorescence staining with Brdu (green) and nestin (red). The arrowheads (white) indicated Brdu/nestin double positive cells (orange). (b) Quantitative analysis of Brdu/nestin double positive cells by Imagine Pro Plus 5.0 in ten nonconsecutive high power microscopic fields (200x). compared with model group at corresponding point in time. Scale bar: 50 μm. (c) Viable new neurons detected by double-labeling immunofluorescence staining with Brdu (red) and NeuN (green). The arrowheads (white) indicated Brdu/NeuN double positive cells (orange). (d) Quantitative analysis of Brdu/NeuN double positive cells by Imagine Pro Plus 5.0 in ten nonconsecutive high power microscopic fields (200x). compared with model group at corresponding point in time. Scale bar: 50 μm.