Ilexonin A Promotes Neuronal Proliferation and Regeneration via Activation of the Canonical Wnt Signaling Pathway after Cerebral Ischemia Reperfusion in Rats

Figure 3

Effect of IA on β-catenin protein and LEF1 mRNA expression levels in ischemic peripheral tissue after ischemic reperfusion. β-catenin protein and LEF1 mRNA were detected by western blotting or RT-PCR and normalized to β-actin as a loading control. Lane 1: control; Lane 2: sham; Lane 3: model 1 d; Lane 4: IA 1 d; Lane 5: model 3 d; Lane 6: IA 3 d; Lane 7: model 7 d; Lane 8: IA 7 d; Lane 9: model 14 d; Lane 10: IA 14 d. (a) β-catenin protein increased at 1 d and reached its peak at 3 d and then reduced gradually. The expression of β-catenin increased after IA treatment compared with model group at corresponding point in time. (b) Quantitative analysis of β-catenin protein. compared with model group at corresponding point in time. (c) LEF1 mRNA increased at 1 d and reached its peak at 3 d and then reduced gradually. The expression of LEF1 mRNA increased after IA treatment compared with model group at corresponding point in time. (d) Quantitative analysis of LEF1 mRNA. compared with model group at corresponding point in time.

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