Antioxidant and Anti-Inflammatory Effects of Herbal Formula SC-E3 in Lipopolysaccharide-Stimulated RAW 264.7 Macrophages

Figure 2

Effects of SC-E3 on ROS generation and on the productions of inflammatory mediators and cytokines in LPS-stimulated RAW 264.7 macrophages. (a) Effects of SC-E3 on viability. Cell viability was determined by MTT assay. Cells were treated with various concentrations of SC-E3 extract (50, 100, 300, or 500 μg/mL) for 24 h. Values were expressed as percentages of the nontreated control. (b) Effect of SC-E3 on ROS generation. Fold increases in intracellular ROS versus nontreated control were determined by measuring DCF fluorescence intensities. (c) Effects of SC-E3 on LPS-induced NO production. Cells were stimulated with 1 μg/mL of LPS, in the absence or presence of various concentrations (50, 100, 300, or 500 μg/mL) of SC-E3 for 18 h. Nitrite production was measured using Griess reagent. (d) Effects of SC-E3 on LPS-induced PGE₂ production. (Significant versus the nontreated control, , significant versus LPS treatment, and .)

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