Effect of Gelsemium elegans and Mussaenda pubescens, the Components of a Detoxification Herbal Formula, on Disturbance of the Intestinal Absorptions of Indole Alkaloids in Caco-2 Cells

Table 1

Precursor/product ion pairs and parameters for MRM of compounds used in this study.

Analyte	GE dichloromethane extract	GM extract	Retention time (min)	[M+H]^+(m/z)	MRM transition (precursor→product)	Peak area of product	Cone voltage (V)	Collision energy (eV)
Gelsemine	+	+	2.65	323.17	323.2→70.0	97565	30	30
					323.2→195.0	17501	30	30
					323.2→236.0	40783	30	25
Humantenidine	+	+	2.72	343.20	343.2→312.1	10351	30	16
					343.2→281.3	1627	30	23
					343.2→108.1	1971	30	25
Koumine	+	+	2.91	307.17	307.2→180.0	78147	20	40
					307.2→167.0	52038	20	50
					307.2→204.0	56263	20	50
Gelsenicine	+	+	3.30	327.17	327.2→296.0	193147	30	25
					327.2→265.0	41147	30	18
					327.2→108.0	23742	30	30
Gelsevirine	+	+	3.37	353.10	353.1→322.0	264565	20	18
					353.1→110.3	29739	20	30
					353.1→259.9	22903	20	30
Humantenine	+	+	4.02	355.11	355.1→309.1	426987	20	35
					355.1→178.3	72919	20	25
					355.1→122.1	142393	20	25

Quantitative ion pair.