Laser Acupuncture Exerts Neuroprotective Effects via Regulation of Creb, Bdnf, Bcl-2, and Bax Gene Expressions in the Hippocampus

Table 2

PCR primer sequences.

Gene	Primer sequence; (forward and reverse)	Product size; (base pair)	Annealing temperature (°C)
Gapdh	5′-TGCATCCTGCACCACCAACT-3′	349	56
	5′-CGCCTGCTTCACCACCTTG-3′
Creb	5′-TACCCAGGGAGGAGCAATAC-3′	183	51
	5′-GAGGCAGCTTGAACAACAAC-3′
Bdnf	5′-CAGGGGCATAGACAAAAG-3′	153	57
	5′-CTTCCCCTTTTAATGGTC-3′
Bcl-2	5′-TTGTGGCCTTCTTTGAGTTCGGTG-3′	168	55
	5′-GGTGCCGGTTCAGGTACTCAGTCA-3′
Box	5′-CCTGTGCACCAAGGTGCCGGAACT-3′	498	55
	5′-CCACCCTGGTCTTGGATCCAGCCC-3′