Research Article
A Chinese Herbal Formula, Gengnianchun, Ameliorates β-Amyloid Peptide Toxicity in a Caenorhabditis elegans Model of Alzheimer’s Disease
Figure 2
GNC reduced the Aβ mRNA and protein levels in C. elegans CL4176. (a) Western blot of the transgenic C. elegans CL4176 treated with GNC (3.94 mg/mL) and the control (H2O). A representative blot of three independent experiments is shown. (b) The mean optical density was calculated using ImageJ. Quantitative comparisons of the Aβ levels depict a significant 35% reduction in Aβ levels () in GNC (3.94 mg/mL) pretreated worms. (c) The GNC (3.94 mg/mL) treatment decreased the levels of the amy-1 transcript by 49% (). β-Actin (act-4) was used as an endogenous control, and the expression levels were determined by qRT-PCR using the method. The data are displayed as means ± SD. ; .
| (a) |
| (b) |
| (c) |