Effects of Shizhifang on NLRP3 Inflammasome Activation and Renal Tubular Injury in Hyperuricemic Rats

Table 1

PCR primer sequence.

Gene name	Primer sequence (5′–3′)	(°C)
TXNIP	F 5′ GGCTTTTCTCGATCGTCCCA 3′	60.11
	R 5′ AGAGGCAGAAAGCGTTGAGT 3′	59.61
NLRP3	F 5′ ACGGCAAGTTCGAAAAAGGC 3′	59.97
	R 5′ AGACCTCGGCAGAAGCTAGA 3′	60.03
ASC	F 5′ AGACATGGGCATACAGGAGC 3′	59.53
	R 5′ GCAATGAGTGCTTGCCTGTG 3′	60.39
Caspase-1	F 5′ AAGAAGGTGGCGCATTTCCT3′	60.25
	R 5′ GACGTGTACGAGTGGGTGTT3′	59.97
IL-1β	F 5′ AGGATTGCTTCCAAGCCCTTGACT 3′	64.44
	R 5′ ACAGCTTCTCCACAGCCACCATGA 3′	66.22
IL-18	F 5′ ACAAAAGAAACCCGCCTGTG 3′	59.26
	R 5′ TGTGTCCTGGCACACGTTTC 3′	61.09
GAPDH	F 5′ TGTGAACGGATTTGGCCGTA3′	59.96
	R 5′ GATGGTGATGGGTTTCCCGT 3′	60.03