Gengnianchun, a Traditional Chinese Medicine, Enhances Oxidative Stress Resistance and Lifespan in Caenorhabditis elegans by Modulating daf-16/FOXO

Figure 3

ROS levels of C. elegans under oxidative stress conditions. Notes: worms were exposed to Cr (VI) for 24 hours and stained with the molecular probe H₂DCF-DA. Images of worms pretreated with the vehicle control (H₂O) are displayed as follows: fluorescent image (a), inverted image (b), and positive signal (c). Worms pretreated with 3.94 mg/mL of GNC are displayed as follows: fluorescent image (d), inverted image (e), and positive signal (f). All images were characterized using Image J. Quantitative comparisons of the ROS levels depict ROS level reductions of 67.95% in GNC-pretreated worms (g, h). .