Compound Schisandra-Ginseng-Notoginseng-Lycium Extract Ameliorates Scopolamine-Induced Learning and Memory Disorders in Mice

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(a) Total 135 mice were randomly divided into nine groups ( each), including one control group administered distilled water and eight treatment groups administered different doses of CSGNL. Mice were administered by gavage once a day 0.1 mL/10 g for 30 days. On days 29 and 30, the step-through test was performed to evaluate the effects of CSGNL dosing ratios. (b) After the optimal dose ratio of CSGNL was determined, mice were divided into five groups of 15 mice each. Group 1 was the control group, and groups 2–5 were administered scopolamine. Groups 1 (control) and 2 (model) received ddH₂O intragastrically. Groups 3–5 were administered by gavage 0.3 CSGNL-L, 0.6 CSGNL-M, and 1.2 g·kg⁻¹ CSGNL-H, respectively, once daily for 30 consecutive days. On days 29 and 30, the step-through test was performed. Ten minutes before the behavioral tests, mice in group 1 were administered normal saline intraperitoneally, and mice in other groups were administered 5 mg·kg⁻¹ scopolamine hydrobromide intraperitoneally to induce memory impairment. (c) Mice were divided into five groups of 18 mice each. Group 1 was the control group, and groups 2–5 were administered scopolamine. Groups 1 (control) and 2 (model) received ddH₂O intragastrically. Groups 3–5 were administered by gavage 0.3 CSGNL-L, 0.6 CSGNL-M, and 1.2 g·kg⁻¹ CSGNL-H, respectively, once daily for 30 consecutive days. Ten minutes before the behavioral tests, mice in group 1 were administered normal saline intraperitoneally, and mice in other groups were administered scopolamine. In each group three mice were used for H&E staining and 15 mice for Morris water maze test as well as AChE, ChAT, and ACh assays. represents the meaning of multiplication.

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