PC2 Ovotransferrin: Characterization and Alternative Immunotherapeutic Activity

Figure 1

Analyzing the OTf values. (a) Agar Gel Immunodiffusion (AGID) Test: the identity establishing between standard OTf (wells 3 and 5) and apo-OTf PC2 (wells 2 and 6); wells 1 and 4 and central well are anti-OTf rabbit IgG. (b) AGID: the identity between holo-OTf (wells 3 and 5) and apo-OTf PC2 (wells 2 and 6); wells 1 and 4 and central well are anti-OTf rabbit IgG. (c) AGID: test for apo-OTf (full OTf and dilutions from 1/2 to 1/32). (d) AGID: test for holo-OTf (full OTf and dilutions from 1/2 to 1/32). (e) The statistical analysis of OTf concentration values (mg/mL) obtained by IDSR test according to the diameter of the precipitation rings (mm) and concentration. The values < and = are indicating a high statistical significance. (f) The standard curve for quantitative determination of OTf by IDSR. (g) Quantification of OTf samples by IDSR: central well: the standard OTf; wells 1 and 2: holo-OTf; wells 3, 4, and 5: apo-OTf and the OTf’s concentration values (mg/mL), depending on the precipitation ring diameters (mm).

(a)

(b)

(c) Apo

(d) Holo

(e)

(f) IDSR: standard curve

(g)