Extracts of Salvia-Nelumbinis Naturalis Ameliorate Nonalcoholic Steatohepatitis via Inhibiting Gut-Derived Endotoxin Mediated TLR4/NF-κB Activation

Figure 7

LPS induced inflammation in Kupffer cells. Kupffer cells were isolated from C57BL/6 mice and identified by fluorescently labeled beads (a). Kupffer cells were treated with LPS (50, 100, and 200 ng/ml) for 2 h, and mRNA expression of TNFα (b) and IL-β (c) in the cells was qualified by qRT-PCR. Kupffer cells were treated with LPS (100 ng/ml), and the cells were collected at different time points (1 h, 2 h, and 4 h). Cellular TNFα (d) and IL-β (e) mRNA expression was analyzed by qRT-PCR. Original magnification of representative images, ×200; data were present as mean ± SE, versus cells cultured with routine medium.

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