Recuperating Lung Decoction Attenuates the Oxidative Stress State of Chronic Obstructive Pulmonary Disease by Inhibiting the MAPK/AP-1 Signal Pathway and Regulating γ-GCS

Figure 5

RLD reduces C-fos, C-jun protein, and mRNA expression in lung tissues. (a) C-fos and C-jun expressions and locations in lung tissues were analyzed by immunohistochemistry . Brown positive expression could be seen at the bronchial wall and alveolar septum. After the RLD and NAC treatment, the positive expressions of proteins were greatly reduced. (b) The C-fos and C-jun proteins expressions were analyzed by Western blot . Both proteins expressions in COPD group were increased. The expression was reduced after RLD treatment. (c) and (d) A semiquantitative analysis on the C-fos and C-jun level in lung tissues and an intergroup comparison were conducted by immunohistochemistry . (e) and (f) A quantitative analysis on the C-fos and C-jun protein in lung tissues and an intergroup comparison were performed by Western blot . The results were consistent with that from immunohistochemistry. (g) and (h) The C-fos and C-jun mRNA levels in tissues were tested by RT-PCR . The C-fos and C-jun mRNA levels in COPD group were increased but were reduced after RLD treatment. The data were expressed as mean ± SD. One-way ANOVA was adopted for statistical analysis. The Newman-Keuls was used for an intergroup comparison. (, , compared with the normal group, , compared with the model group; ).

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