Cytotoxicity and Apoptosis Induction of Ardisia crispa and Its Solvent Partitions against Mus musculus Mammary Carcinoma Cell Line (4T1)

Figure 3

4T1 cells stained with dual staining AO/PI following treatment with 80% methanol extract for 72 hours. The cells were viewed under inverted research fluorescence microscope (Nikon ECLIPSE Ti-S, Shinagawa-ku, Tokyo, Japan) at 20x magnification. (a) Control (untreated 4T1 cells) being majorly viable and uniform in shape. Green fluorescent colour indicates cells are viable. (b) 4T1 cells treated with 42.26 μg/mL of 80% methanol extract. Red colour indicates cells that had undergone necrosis while orange colour indicates cells that had undergone apoptosis. Number of viable cells was reduced and cells were showing shrinkage. Scale bars represent 100 μm.

(a)

(b)