Research Article

Digital Gene Expression Profiling Analysis of Aged Mice under Moxibustion Treatment

Table 1

Summary of sequencing analysis.

ItemC1C2C3M1M2M3

Raw reads26,827,940 (100%)22,683,746 (100%)21,586,308 (100%)21,008,620 (100%)25,584,692 (100%)27,045,698 (100%)
Clean reads25,927,296 (96.64%)21,924,166 (96.65%)20,808,688 (96.40%)20,280,672 (96.54%)24,686,554 (96.49%)26,115,966 (96.56%)
Clean bases3.89 G3.29 G3.12 G3.04 G3.70 G3.92 G
Error rate0.02%0.02%0.02%0.02%0.02%0.02%
Q2097.27%97.24%97.07%97.2%97.12%97.19%
Q3092.97%92.92%92.63%92.86%92.7%92.84%
GC content50.55%50.32%50.86%50.59%50.34%50.34%

C1, C2, and C3: control group; M1, M2, and M3: moxibustion group. Q20: the percentage of bases with a Phred value > 20; Q30: the percentage of bases with a Phred value > 30.