Research Article
Total Flavonoids from Radix Glycyrrhiza Exert Anti-Inflammatory and Antitumorigenic Effects by Inactivating iNOS Signaling Pathways
Figure 2
Effect of TFRG on iNOS expression in LPS/IFNγ-stimulated RAW264.7 cells. (a) RAW 264.7 cells (1 × 106 cells/dish) were pretreated with varying concentrations of TFRG for 1 h followed by LPS (100 ng/ml) and IFN-γ (10 U/ml) treatment for 4 h. Total RNA was isolated and subjected to qRT-PCR. GAPDH mRNA was used as an internal control for standardization. (b) Cells were plated at a density of 1 × 106 cells/well in 30-mm dishes and allowed to attach overnight. TFRG (20, 40, 60 μg/ml) was added 1 h prior to the treatment of IFNγ (10 U/ml) plus LPS (100 ng/ml) for 6 h. Whole cells lysates were analyzed and standardized by protein concentration. The data shown are representative of three independent experiments. versus control group; versus model group.
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