Prevention of Prostate Tumor Development by Stimulation of Antitumor Immunity Using a Standardized Herbal Extract (Deep Immune®) in TRAMP Mice

Figure 4

The DI activates the tumor-killing activity in cocultures of tumor cells with immune cells (macrophages or splenocytes). (a) RAW264.7 cells were cocultured with mouse prostate tumor cells (TRAMP-C2) (1 : 1, RAW264.7 : TRAMP-C2 with extract ratio) or without target cells (0 : 1, TRAMP-C2 with extract only). The data were presented as mean ± SD of each treatment ( compared to “0” control, one-way ANOVA, –20). (b) The splenocytes from different tumor-bearing TRAMP mice (20–35 weeks old) were cocultured with mouse prostate tumor cells (TRAMP-C2) (1 : 1 ratio). The data were presented as mean of 6 determinants at each treatment for each mouse ( compared to “0” control group, one-way ANOVA, ). The cultures were stimulated with herbal extract at different concentrations (0, 25, 50, or 100 μg/mL) for 24 h. The cytotoxicity was determined by the percentage of Calcein-AM release in Calcein-AM release assay.

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