Antiplatelet and Antithrombotic Activities of Lespedeza cuneata via Pharmacological Inhibition of Integrin αIIbβ3, MAPK, and PI3K/AKT Pathways and FeCl3-Induced Murine Thrombosis

Figure 5

L. cuneata inhibits collagen-stimulated platelet intracellular calcium ion ([Ca²⁺]_i) mobilization and ATP release from alpha granules. (a) The amount of intracellular calcium release was quantified by using this formula, [Ca²⁺]_i = 224 nM (F )/( F), where and are the fluorescence intensities at extremely low and very high Ca²⁺ concentrations, respectively, and F is the dissociation constant of the Fura-2-Ca²⁺complex. (b) ATP release in collagen-stimulated rat platelets was measured using supernatant from the aggregation reaction using an ATP ELISA kit. , , and compared with the agonist.

(a)

(b)