Antiplatelet and Antithrombotic Activities of Lespedeza cuneata via Pharmacological Inhibition of Integrin αIIbβ3, MAPK, and PI3K/AKT Pathways and FeCl3-Induced Murine Thrombosis

Figure 6

Assessment of integrin αIIbβ3-mediated inside-out signaling in the presence of Lespedeza cuneata. In the presence of an antifibrinogen antibody, washed platelets were pretreated with the plant extract and activated with an agonist for 5 min. After that, paraformaldehyde 0.5% was used to fix the platelets. A flow cytometer (FACS Aria III) was used to assess integrin αIIbβ3 activation which was significantly induced by collagen and reduced dose dependently by the dose of treatment. , , and compared with the agonist-treated group.