Antiplatelet and Antithrombotic Activities of Lespedeza cuneata via Pharmacological Inhibition of Integrin αIIbβ3, MAPK, and PI3K/AKT Pathways and FeCl3-Induced Murine Thrombosis

Figure 7

Assessment of clot retraction by outside-in signaling in the presence of Lespedeza cuneata. After incubating PRP (250 L) with the vehicle, L. cuneata, or Y27632 (rock inhibitor) for 2 min, the volume was increased to 1 mL by adding RBCs (5 L) and Tyrode’s buffer. The injection of thrombin (1 U/mL) caused the clot to retract, which was then monitored for 90 min at room temperature. To evaluate clot retraction, clot weight was lastly assessed. and vs. thrombin (THR).