Antiplatelet and Antithrombotic Activities of Lespedeza cuneata via Pharmacological Inhibition of Integrin αIIbβ3, MAPK, and PI3K/AKT Pathways and FeCl3-Induced Murine Thrombosis

Figure 9

L. cuneata prevents thrombosis and regulates hemostasis. To evaluate FeCl₃-induced thrombus formation and the tail bleeding assay, four treatment groups were treated orally for 7 days (saline, ASA, or a low and high dose of the 50% EtOH extract of L. cuneata) using C57BL/6J and ICR mice, respectively. Mice were anesthetized, and FeCl₃-induced thrombus formation and the tail bleeding assay were carried out almost 1 h after the last treatment. compared with the agonist.