]>Rosiglitazone Prevents High Glucose-Induced Vascular Endothelial Growth Factor and Collagen IV Expression in Cultured Mesangial Cells : Figure 5
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Figure 5: Rosiglitazone prevented high glucose-induced collagen IV expression . Mesangial cells were exposed to 5.6 mM (NG) or 25 mM (HG) for up to 48 hours pretreated with 10  𝜇 M rosiglitazone (RSG) for 1 hour. (a) The expression of collagen IV ( 𝛼 1) mRNA was detected by real-time RT-PCR ( 𝑛 = 4 , ∗ 𝑃 < . 0 5 versus NG). (b) Representative confocal microscopic images show collagen IV staining without (C) or with RSG. Immunofluorescence intensity per cell was analyzed ( 𝑛 = 7 6 - 1 3 6 cells, ∗ ∗ 𝑃 < . 0 1 versus NG). Magnification bar = 25  𝜇 M.