Fetoplacental Vascular Endothelial Dysfunction as an Early Phenomenon in the Programming of Human Adult Diseases in Subjects Born from Gestational Diabetes Mellitus or Obesity in Pregnancy
Figure 6
Insulin effect on human umbilical vein ring reactivity. Endothelium-intact human umbilical vein rings were isolated from umbilical veins taken from pregnancies with maternal physiological hypercholesterolaemia (MPH) or maternal supraphysiological hypercholesterolaemia (MSPH). Umbilical vessel ring segments (2–4 mm length) were mounted in a myograph for isometric force measurements with optimal diameter adjusted from maximal active response to 62.5 mM KCl as previously described [28, 107]. Acute response to insulin (3 minutes) was determined in KCl-preconstricted vessels in preparations incubated in Krebs. Values are mean ± SEM (). * versus corresponding values in MPH.
