Clinical Specimens are the Pool of Multidrug- resistant Pseudomonas aeruginosa Harbouring oprL and toxA Virulence Genes: Findings from a Tertiary Hospital of Nepal

Table 1

Nucleotide sequence of primers and condition used to amplify species-specific virulence genes in P. aeruginosa by PCR.

Virulence factors	Target genes	Primer names	Sequence (5′ to 3′)	Annealing temperature (°C)	Amplicon size (bp)	Refs
Exotoxin A	toxA	toxA-f	GGTAACCAGCTCAGCCACAT	58.2	352	[34]
		toxA-r	TGATGTCCAGGTCATGCTTC
Sialidase enzyme	oprL	oprL-f	ATGGAAATGCTGAAATTCGGC	61.8	504	[18]
		oprL-r	CTTCTTCAGCTCGACGCGACG