Expression, Purification, and Characterisation of Dehydroquinate Synthase from Pyrococcus furiosus

<table>Sequence alignment for PfuDHQS and EcoDHQS with characterised DHQSs (Ani: A. nidulans DHQS, Hpy: H. pylori DHQS, Eco: E. coli DHQS, Pfu: P. furiosus DHQS, Tth: T. thermophilus DHQS, Sau: S. aureus DHQS). Residues highlighted in yellow are metal binding ligands. Residues associated with substrate binding are highlighted in green, and cofactor NAD+ binding residues are highlighted in blue.</table>

Enzyme Research

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Figure 2

Figure 2: Expression, Purification, and Characterisation of Dehydroquinate Synthase from Pyrococcus furiosus