Expression, Purification, and Characterisation of Dehydroquinate Synthase from Pyrococcus furiosus

Figure 6

Overlay of modelled PfuDHQS with the structures of A. nidulans DHQS and T. thermophilus DHQS. PfuDHQS was modelled using PRIME 1.5 based on the structure of S. aureus DHQS (1XAI). (a) Overlay of modelled PfuDHQS (yellow) with A. nidulans (1SG6, magenta). (b) Overlay of modelled PfuDHQS (yellow) with T. thermophilus DHQS (1UJN, cyan). Structures of the ligands, NAD⁺ and the carbaphosphonate analogue of DAH7P, are shown by green sticks (from 1SG6). The arrows indicate the position of the N-terminus (top) and the loop around Glu194, where loops are predicted to be considerably shorter in PfuDHQS.

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