Research Article
Enhancement of Cellulase Activity from a New Strain of Bacillus subtilis by Medium Optimization and Analysis with Various Cellulosic Substrates
Table 1
Plackett-Burman design in coded units and real values (in parenthesis) in g/L for seven variables along with the cellulase activity.
| Run
Order | CMC | Peptone | YE | K2HPO4 | MgSO4·7H2O () | FeSO4·7H2O () | MnCl2·4H2O () | E.A (U/mL) |
| | (1) | +1 (18) | −1 (2) | −1 (1) | −1 (0.5) | −1 (0.05) | +1 (0.45) | −1 (0.01) | 0.084 | | (2) | −1 (2) | −1 (2) | −1 (1) | −1 (0.5) | +1 (0.45) | −1 (0.05) | +1 (0.1) | 0.003 | | (3) | −1 (2) | +1 (8) | +1 (9) | +1 (2) | +1 (0.45) | −1 (0.05) | −1 (0.01) | 0.080 | | (4) | +1 (18) | −1 (2) | +1 (9) | +1 (2) | −1 (0.05) | −1 (0.05) | −1 (0.01) | 0.226 | | (5) | −1 (2) | −1 (2) | −1 (1) | +1 (2) | −1 (0.05) | +1 (0.45) | −1 (0.01) | 0.004 | | (6) | +1 (18) | +1 (8) | +1 (9) | +1 (2) | −1 (0.05) | −1 (0.05) | +1 (0.1) | 0.252 | | (7) | +1 (18) | +1 (8) | −1 (1) | +1 (2) | +1 (0.45) | −1 (0.05) | −1 (0.01) | 0.221 | | (8) | −1 (2) | +1 (8) | +1 (9) | −1 (0.5) | +1 (0.45) | +1 (0.45) | −1 (0.01) | 0.026 | | (9) | +1 (18) | −1 (2) | +1 (9) | +1 (2) | +1 (0.45) | +1 (0.45) | −1 (0.01) | 0.205 | | (10) | −1 (2) | −1 (2) | −1 (1) | −1 (0.5) | −1 (0.05) | −1 (0.05) | −1 (0.01) | 0.006 | | (11) | −1 (2) | +1 (8) | +1 (9) | −1 (0.5) | −1 (0.05) | −1 (0.05) | −1 (0.01) | 0.049 | | (12) | +1 (18) | +1 (8) | −1 (1) | −1 (0.5) | +1 (0.45) | +1 (0.45) | −1 (0.01) | 0.168 | | (13) | +1 (18) | −1 (2) | −1 (1) | +1 (2) | +1 (0.45) | −1 (0.05) | +1 (0.1) | 0.102 | | (14) | +1 (18) | −1 (2) | +1 (9) | −1 (0.5) | +1 (0.45) | +1 (0.45) | +1 (0.1) | 0.197 | | (15) | −1 (2) | −1 (2) | +1 (9) | −1 (0.5) | +1 (0.45) | −1 (0.05) | +1 (0.1) | 0.024 | | (16) | −1 (2) | +1 (8) | −1 (1) | +1 (2) | +1 (0.45) | +1 (0.45) | +1 (0.1) | 0.003 | | (17) | +1 (18) | +1 (8) | −1 (1) | −1 (0.5) | −1 (0.05) | −1 (0.05) | +1 (0.1) | 0.214 | | (18) | +1 (18) | +1 (8) | +1 (9) | −1 (0.5) | −1 (0.05) | +1 (0.45) | +1 (0.1) | 0.249 | | (19) | −1 (2) | +1 (8) | −1 (1) | +1 (2) | −1 (0.05) | +1 (0.45) | +1 (0.1) | 0.025 | | (20) | −1 (2) | −1 (2) | +1 (9) | +1 (2) | −1 (0.05) | +1 (0.45) | +1 (0.1) | 0.022 |
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