Assessment of the Morphological, Biochemical, and Kinetic Properties for Candida rugosa Lipase Immobilized on Hydrous Niobium Oxide to Be Used in the Biodiesel Synthesis

Table 3

Half-life (t _1/2) and rate of denaturation (𝑘_𝑑) for the free and immobilized lipases.

Temperature (°C)	Rate of denaturation (𝑘𝑑) (h^−1)		Half-life (𝑡1/2)=−ln(1/2)/𝑘𝑑 (h)
	Free lipase	Immobilized lipase	Free lipase	Immobilized lipase
40	0.23	0.22	3.03	3.15
50	1.15	0.52	0.60	1.36
55	1.81	0.84	0.38	0.82