Isolation, Purification, and Characterization of Fungal Laccase from Pleurotus sp.

Table 1

Summary of purification of laccase from Pleurotus sp.

Purification step	Volume (mL)	Protein (mg)	Total activity (U)	Specific activity (U/mg)	Purification (fold)	Yield (%)
Culture filtrate	800	2300	29000	36	1	100
Ammonium sulfate fraction	60	1300	18000	107	2.97	62
DEAE Cellulose chromatography	15	125	14500	1300	12.14	50
Sephadex G-100 chromatography	8	12	6500	2600	72.2	22.4