Inhibitory Effect of Phthalic Acid on Tyrosinase: The Mixed-Type Inhibition and Docking Simulations

<table>Plot of maximum fluorescence intensity versus [PA]. (a) Tyrosinase was incubated with various concentrations of PA (1.56 to 800 mM). (b) Double reciprocal plot of <svg height="14.75" id="M39" style="vertical-align:-3.25793pt" version="1.1" viewbox="0 0 73.6875 14.75" width="73.6875" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,14.75)">
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<text transform="matrix(1,0,0,-1,-59.35,63.5)">
<tspan style="font-size: 12.50px; " x="0" y="0">/</tspan>
<tspan style="font-size: 12.50px; " x="3.4758339" y="0">(</tspan>
<tspan style="font-size: 12.50px; " x="7.6393328" y="0">𝐹</tspan>
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<text transform="matrix(1,0,0,-1,-43.98,60.37)">
<tspan style="font-size: 8.75px; " x="0" y="0">0</tspan>
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<text transform="matrix(1,0,0,-1,-36.33,63.5)">
<tspan style="font-size: 12.50px; " x="0" y="0">−</tspan>
<tspan style="font-size: 12.50px; " x="11.340221" y="0">𝐹</tspan>
<tspan style="font-size: 12.50px; " x="19.067076" y="0">)</tspan>
</text>
</g>
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</svg> versus <svg height="16.275" id="M40" style="vertical-align:-1.95624pt" version="1.1" viewbox="0 0 36.325001 16.275" width="36.325001" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,16.275)">
<g transform="translate(72,-58.98)">
<text transform="matrix(1,0,0,-1,-71.95,60.98)">
<tspan style="font-size: 12.50px; " x="0" y="0">[</tspan>
<tspan style="font-size: 12.50px; " x="4.1634989" y="0">𝑄</tspan>
<tspan style="font-size: 12.50px; " x="13.928342" y="0">]</tspan>
</text>
<text transform="matrix(1,0,0,-1,-53.86,66.03)">
<tspan style="font-size: 8.75px; " x="0" y="0">−</tspan>
<tspan style="font-size: 8.75px; " x="5.99512" y="0">1</tspan>
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</svg>. Data was treated according to (<a href="https://static.hindawi.com/articles/er/volume-2011/294724/figures/#EEq4">4</a>). <svg height="14.3875" id="M41" style="vertical-align:-3.25793pt" version="1.1" viewbox="0 0 15.875 14.3875" width="15.875" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,14.3875)">
<g transform="translate(72,-60.49)">
<text transform="matrix(1,0,0,-1,-71.95,63.79)">
<tspan style="font-size: 12.50px; " x="0" y="0">𝐹</tspan>
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<tspan style="font-size: 8.75px; " x="0" y="0">0</tspan>
</text>
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</svg> maximum native fluorescence intensity; <svg height="10.325" id="M42" style="vertical-align:-0.0pt" version="1.1" viewbox="0 0 12.05 10.325" width="12.05" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,10.325)">
<g transform="translate(72,-63.74)">
<text transform="matrix(1,0,0,-1,-71.95,63.79)">
<tspan style="font-size: 12.50px; " x="0" y="0">𝐹</tspan>
</text>
</g>
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</svg> maximum fluorescence intensity of sample; <svg height="12.925" id="M43" style="vertical-align:-1.90608pt" version="1.1" viewbox="0 0 12.325 12.925" width="12.325" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,12.925)">
<g transform="translate(72,-61.66)">
<text transform="matrix(1,0,0,-1,-71.95,63.61)">
<tspan style="font-size: 12.50px; " x="0" y="0">𝑄</tspan>
</text>
</g>
</g>
</svg> quencher PA.</table>

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fig6

Figure 6

Figure 6: Inhibitory Effect of Phthalic Acid on Tyrosinase: The Mixed-Type Inhibition and Docking Simulations 