Application of Asymetrical and Hoke Designs for Optimization of Laccase Production by the White-Rot Fungus Fomes fomentarius in Solid-State Fermentation

Table 1

Experimental conditions of the asymmetrical screening design.

	Levels
Factors	1	2	3
M/S ratio (ml/gs) (A)	3	6	—
MgSO4 (mg/gs) (B)	0.3	3	—
CaCl2 (mg/gs) (C)	0.3	3	—
Moisturizing agent* (D)	Sodium acetate; buffer (SAB)	Citrate phosphate; buffer (CPB)	—
Inoculum size (discs**) (E)	4	8	—
Glucose (mg/gs) (F)	0	30	60
Ammonium tartrate (mg/gs) (G)	0	5.5	11
CuSO4 (ml/gs) (H)	0	0.75	1.5

*20 mM, pH 5; **diameter, 6 mm.