Laccase-Based CLEAs: Chitosan as a Novel Cross-Linking Agent

Table 1

Chitosan and EDAC concentrations tested for the optimization of the CLEAs formation at 4°C during 48 hours.

Experimental design type	Sample names	Chitosan concentration (g/L)	EDAC concentration (mM)
3^2	CLEA-0.2-200	0.2	200
	CLEA-0.2-400	0.2	400
	CLEA-0.2-600	0.2	600
	CLEA-0.6-200	0.6	200
	CLEA-0.6-400	0.6	400
	CLEA-0.6-600	0.6	600
	CLEA-1.0-200	1.0	200
	CLEA-1.0-400	1.0	400
	CLEA-1.0-600	1.0	600
Modified Central Composite	CLEA-0.5-1	0.5	1
	CLEA-0.5-100	0.5	100
	CLEA-1.5-1	1.5	1
	CLEA-1.5-100	1.5	100
	CLEA-1.0-0.05	1.0	0.05
	CLEA-1.0-50.5	1.0	50.5
	CLEA-1.0-136	1.0	136
	CLEA-0.134-50.5	0.134	50.5
	CLEA-1.867-50.5	1.867	50.5