Laccase-Based CLEAs: Chitosan as a Novel Cross-Linking Agent

Table 5

Michaelis-Menten kinetic constants of laccase CLEAs for the oxidation of ABTS^a.

Sample	Km (mM)	Kcat (μmol/s/mg)	Kcat/Km (L⋅mg/s)
CLEA-1.0-136	0.083 ± 0,015	30.760 ± 0.900	0.369
CLEA-1.5-100	0.156 ± 0,026	2.524 ± 0.091	0.016
CLEA-1.867-50.5	0.259 ± 0,044	0.986 ± 0.046	0.004
CLEA-1.0-50.5	0.101 ± 0,027	5.295 ± 0.250	0.053
Free laccase	0.082 ± 0,010	2.694 ± 0.060	0.033

^aResults are mean of triplicate measures ± standard deviation.