The Involvement of Glutamate Metabolism in the Resistance to Thermal, Nutritional, and Oxidative Stress in Trypanosoma cruzi

Figure 6

The combined effect of starvation, oxidative stress, and analogues treatments on epimastigotes. Parasites previously incubated in PBS (PBS), 3 mM L-proline in PBS (PRO), or 3 mM glucose in PBS (GLC), and subjected either to oxidative stress for 3 h by addition of 80 μM H₂O₂ or to water, were treated with or without (control) 20 mM MS (a), 60 mM MSE (b), or 50 mM MSO (c). After washing, the parasites were incubated for 3 h in PBS and stressed by the addition of 80 μM H₂O₂. The cell viability was evaluated by MTT assay.

(a)

(b)

(c)