Keratinase Production by Three Bacillus spp. Using Feather Meal and Whole Feather as Substrate in a Submerged Fermentation
Figure 6
Effect of proteolytic inhibitors on the extracellular gelatinases (a) and keratinases (b) of Bacillus sp. grown in feather medium for 7 days at 28°C. Gel strips containing concentrated culture supernatant were incubated for 48 h at 37°C in proteolysis buffer (0.1 M citric acid buffer pH 5.0) in the absence (control) or in the presence of different proteolytic inhibitors: 3 mmoL l−1 phenylmethylsulfonyl fluoride (PMSF), 0.26 mmoL l−1 ethylenediaminetetraacetic acid (EDTA), 10 mmoL l−1 1,10 phenanthroline (Phenan), 10 μmoL l−1 pepstatin A (Peps), and 5 μmoL l−1 M transepoxysuccinyl L-leucylamido-(4-guanidino) butane (E-64).
