Enzymatic Synthesis of the Flavone Glucosides, Prunin and Isoquercetin, and the Aglycones, Naringenin and Quercetin, with Selective 𝜶-L-Rhamnosidase and 𝜷-D-Glucosidase Activities of Naringinase

Figure 6

TLC of (a) naringin (Rf = 0.62), (b) prunin (Rf = 0.58), (c) naringenin (Rf = 0.30), (d) rutin (Rf = 0.55), (e) isoquercetin (Rf = 0.53), and (f) quercetin (Rf = 0.30). All of compounds were visible with UV light (254 nm). After acid revelation + heating (150°C), naringin, prunin, and naringenin showed orange spots and rutin, quercetin, and isoquercetin, yellow.