Research Article

Enzymatic Synthesis of the Flavone Glucosides, Prunin and Isoquercetin, and the Aglycones, Naringenin and Quercetin, with Selective -L-Rhamnosidase and -D-Glucosidase Activities of Naringinase

Table 5

Second-order model equations for the response surfaces fitted to the experimental data points of α-L-rhamnosidase residual activity ( ), on first deactivation rate coefficient ( ) for α-L-rhamnosidase and β-D-glucosidase activities of naringinase, as a function of temperature (T) and pH, and respective and .

Coefficient P P P
(α-L-rhamnosidase)(α-L-rhamnosidase)(β-D-glucosidase)

−63.6717 0.00044.85410.0068178.45160.0419
Linear
1.56450.0004 −0.1193 0.0064 −4.2067 0.0456
0.36610.0322 −0.1579 0.0436 −7.0252 0.1032
Quadratic
−0.0095 0.00040.000770.00550.02630.0443
−0.0123 0.03550.010560.00390.49610.0106
Cross-product
0.00330.05300.000790.30560.031860.4793
0.9360.98120.9047
0.8720.96240.8095