A Guided Tour of the Structural Biology of Gaucher Disease: Acid-β-Glucosidase and Saposin C

Figure 3

Active sites from different GCase structures. (a) Superposition of structures presented in (b)–(h) with residues labeled and any ligands omitted. (b) Apo GCase, (c) sulfate-bound GCase (PDB code 1OGS), (d) glycerol-bound GCase (PDB code 1NT1), (e) CBE-bound GCase (PDB code 1Y7V), (f) IFG-bound GCase (PDB code 1NT1), (g) NB-DNJ (PDB code 2V3D), and (h) N-octyl(cyclic guanidine)-nojirimycin (PDB code 2WCG). Dashed lines indicate hydrogen bonding interactions (2.5–3.5 Å distance from N, O atoms).

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)